Methods and reagents for live-cell gene expression quantification
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United States of America Patent
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Issued Date -
May 22, 2003
app pub date -
Sep 28, 2001
filing date -
Sep 28, 2000
priority date (Note) -
Abandoned
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Abstract
The present invention provides reagents and methods for mRNA quantification in intact cells involving providing cells that possess a target gene of interest that has been tagged with a binding site for an RNA binding protein, and a fluorescently labeled RNA binding polypeptide that includes an RNA binding domain that binds to the binding site, and calculating a quantity of expression of the target gene in the cells using fluorescence signaling techniques.
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