Artificial chromosomes, uses therof and methods for preparing artificial chromosomes
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United States of America Patent
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Issued Date -
May 29, 2003
app pub date -
Nov 1, 2002
filing date -
Apr 10, 1996
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
Methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells and tissues are provided. In particular, satellite artificial chromosomes that, except for inserted heterologous DNA, are substantially composed of heterochromatin are provided. Methods for use of the artificial chromosomes, including for production of transgenic plants, are also provided.
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