Gene silencing using sense DNA and antisense RNA hybrid constructs
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United States of America Patent
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Issued Date -
Jun 5, 2003
app pub date -
Jan 18, 2002
filing date -
Nov 12, 2001
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Abandoned
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Abstract
The present invention provides a stable and efficient method for stimulating RNAi-related gene silencing effects. The present invention also provides a fast, simple and specific method for generating amplified cDNA-aRNA hybrids whose quantity and quality are high enough to be used in specific gene silencing transfection. This improved RNA-polymerase cycling reaction (RNA-PCR) relies upon a thermocycling procedure of in-vitro transcription and reverse transcription to bring up the amount of DNA-RNA hybrids up to two thousand folds within one round of the reaction. The resulting cDNA-aRNA product is useful for silencing either endogenous or exogenous gene expression in transfected cells.
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