Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
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United States of America Patent
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Issued Date -
Jun 5, 2003
app pub date -
Apr 25, 2002
filing date -
Aug 12, 1998
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Abandoned
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Abstract
Method of gene shuffling using oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
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