Factor VIII separation
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United States of America Patent
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Dec 5, 2006
Grant Date -
Jun 12, 2003
app pub date -
Jul 15, 2002
filing date -
Jul 13, 2001
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The claims describe methods for isolating functionally active Factor VIII using a membrane-based separation system containing a separation membrane to create a first and second interstitial volume between at least two restriction membranes. One or more stabilizing agents are added to the sample and/or an interstitial volume. A solvent in the first interstitial volume maintains FVIII in a desired charge state. Applying a potential between the first and second interstitial volumes separates FVIII on one side of the separation membrane from unwanted molecules on the other side of the separation membrane. These methods may also be used as a substitute for one or more steps in a conventional purification scheme for the separation of native or recombinant FVIII.
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