Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts
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United States of America Patent
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Issued Date -
Jun 12, 2003
app pub date -
Jul 2, 2002
filing date -
Jul 3, 2001
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Abandoned
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Abstract
A real-time RT-PCR assay is disclosed that is capable of t(15;17) fusion transcript relative quantitation and simultaneous bcr identification. This assay uses one-step chemistry, incorporates a multiplexed endogenous control and uses a novel dual probe technique to achieve in two simple reactions what has traditionally required a laborious procedure of three or more reaction followed by post-amplification analysis.
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