Methods and reagents for identifying synthetic genetic elements
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United States of America Patent
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Issued Date -
Aug 21, 2003
app pub date -
Sep 12, 2001
filing date -
Mar 12, 1999
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Abandoned
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Abstract
The present invention relates to a selection method that allows fast recovery and identification of functional gene fragments which selectively inhibit growth, e.g., are cytostatic or cytotoxic, of particular cell-types, such as transformed cells. The strategy relies, in part, on the ability of small gene fragments to encode dominant-acting synthetic genetic elements (SGEs), e.g., molecules which interfere with the function of genes from which they are derived. SGEs which can be identified by the subject method include, but are not limited to, inhibitory antisense RNA molecules, ribozymes, nucleic acid decoys, and small peptides.
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