Stabilisation of plasmid inheritance in bacteria
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United States of America Patent
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app pub date -
Apr 3, 2003
filing date -
Oct 3, 2000
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Abandoned
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Abstract
The invention relates to a vaccine comprising a pharmaceutically acceptable carrier or diluent and a bacterium, wherein the bacterium contains (i) a DNA sequence encoding a site-specific recombinase, and (ii) a plasmid comprising a recognition element for the recombinase and a DNA sequence encoding a heterologous polypeptide. The recombinase stabilises inheritance of the plasmid and thereby prevents the bacterium from losing the ability to express the heterologous polypeptide.
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