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Abstract

An electrophoresis separation material, especially for isoelectric focusing, comprising a carrier material to which pH-buffering groups are firmly attached and anode side and cathode sides. At least a first portion of the pH-buffering groups (a) having a pH-dependent charge on a nitrogen atom binding to an sp2-hybridised carbon atom,(b) including groups with pKa.gtoreq.9.5. One embodiment, the groups in (a) have the structure --(NH).sub.nC(.dbd.NH)(NH2)(I), possibly in protonated form, where n is 0 or 1, and the free valence binds to the carrier material via an organic linker. An IEF separation material obtained by polymerisation of a mixture of monomers and containing immobilised buffering groups capable of establishing an immobilised pH-gradient where(1) at least one monomer has the structure CH2.dbd.CHR'CONR.sub.12R.sub.13 (V) where (i) R' represents hydrogen or methyl; (ii) one or more of R.sub.12 and R.sub.13 are --((CH.sub.2).sub.n'--O).sub.n'--H where n' is 2-3 and n' is 1-5 while any remaining group R.sub.12 and R.sub.13, is hydrogen and when n is 3, a hydrogen on the middle carbon in (CH.sub.2).sub.n may be replaced with hydroxy; and (2) the immobilised pH gradient extends to pH>10.

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First Claim