Long-lived keratinocytes
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United States of America Patent
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Issued Date -
Nov 27, 2003
app pub date -
May 24, 2002
filing date -
May 24, 2002
priority date (Note) -
Abandoned
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Abstract
The invention features methods of producing a preparation of very long lived epithelial cells, e.g., keratinocytes. The method includes providing a human epithelial tissue, e.g., epidermis; isolating at least one keratinocyte clone from the tissue; and determining if the clone is capable of at least 100, 150, 200, 250, 300, 350 or 400 population doublings after isolation from human tissue. Preparations of very long lived keratinocytes and methods of using the keratinocytes are also provided.
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