Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
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Issued Date -
Nov 27, 2003
app pub date -
Mar 10, 2003
filing date -
Dec 15, 1997
priority date (Note) -
Abandoned
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Abstract
A homogeneous assay that utilizes molecular beacons as the reporter and nucleic acid ligands as the sensor is described. This assay, called the ligand beacon assay, is for the detection of target molecules in a test mixture. The concept of the ligand beacon assay was tested using several proteins to which high affinity and specific nucleic acid ligands are available. The assay specifically detects the molecular target that binds the nucleic acid ligand with high affinity and specificity. The range of the assay is dictated by the concentration of the nucleic acid ligand/ligand beacon pair used in the assay. Target proteins were detected in buffer as well as in plasma, expanding its applicability to clinical use. This is a simple to use and fast assay format with the potential for automation for high throughput screening applications.
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Patent Owner | Address | |
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GILEAD SCIENCES INC | 333 LAKESIDE DRIVE FOSTER CITY CA 94404 |
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Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
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Gold, Larry | Boulder, CO | 241 | 11499 |
Jayasena, Sumedha | Boulder, CO | 26 | 965 |
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Surcharge after expiration - Late payment is unavoidable | $700.00 | $350.00 | $175.00 |
Surcharge after expiration - Late payment is unintentional | $1,640.00 | $820.00 | $410.00 |
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