Fluorescence illumination optimization for three-dimensional microscopy
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United States of America Patent
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Issued Date -
Jan 15, 2004
app pub date -
Jun 11, 2003
filing date -
Jun 11, 2002
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A fluorescence microscopy system and method allow selective and repeatable switching between Kohler illumination, providing a relatively large field of view, and Critical Illumination, providing a relatively higher axial resolution. This switching or toggle function may be facilitated by a feature associated with an alignment module that allows the focus of the fiber tip, for example, to be selectively and repeatably transitioned between the back aperture of the objective lens and the object plane without losing optical alignment.
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