Method for the quantification of carbohydrates
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United States of America Patent
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Issued Date -
Feb 5, 2004
app pub date -
Aug 28, 2003
filing date -
Nov 9, 2000
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention relates to a method for quantification of carbohydrates in two or more starting samples. The method comprises the steps of: (i) providing a combined sample containing for each carbohydrate a mixture of mass tagged form(s) derived from the carbohydrate, wherein each of said one or more mass tagged forms in the mixture: comprises a mass tag unique for the starting sample from which its carbohydrate part is derived, and is present in the combined sample in an amount that relates to the amount of the carbohydrate in the starting sample from which its carbohydrate part is derived; (ii) subjecting each mixture to mass spectrometry to obtain a mass spectrum; (iii) quantifying the amount of a carbohydrate to be quantified in one original sample relative to the amount(s) of the same carbohydrate in one or more of the other original samples. The invention also relates to a kit of reagents useful in the method above.
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