Systems and methods for detection of nuclear receptor function using reporter enzyme mutant complementation
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United States of America Patent
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Issued Date -
Feb 12, 2004
app pub date -
Mar 25, 2003
filing date -
Mar 25, 2002
priority date (Note) -
Abandoned
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Abstract
Systems and methods for detecting and assaying nuclear receptor activity and screening for nuclear receptor ligands, cofactors, and other compounds that interact with components of the nuclear receptor regulatory process are provided. The assay systems and methods employ complementary protein fused enzyme fragment technology to assay nuclear receptor activity. These methods have application for nuclear receptor ligand and cofactor screening. In particular, these techniques can be used to find ligands for orphan nuclear receptors and to determine the function of orphan nuclear receptors. In this manner, the methods of the invention can be used to find new drugs.
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