Apparatus and method for drug discovery based on intrinsic protein fluorescence
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United States of America Patent
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Issued Date -
Feb 12, 2004
app pub date -
Sep 27, 2002
filing date -
Sep 28, 2001
priority date (Note) -
Abandoned
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Abstract
A phase fluorometer apparatus and method for protein dynamics characterization where a sample is excited with a polarized light, sinusoidally modulated at a single frequency, and the phase difference between polarization components of the emission is detected and processed. The phase difference depending on the modulation frequency, the rate of fluorophore rotation, and the freedom and isotropy of these rotations. The apparatus and method allows information to be collected on the time dependence of the emission anisotropy of a protein of interest and further allows for a ranking of proteins relative to each other.
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