Circular probe amplification (cpa) using energy-transfer primers
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United States of America Patent
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Issued Date -
Apr 8, 2004
app pub date -
Aug 22, 2003
filing date -
Feb 27, 2001
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention provides methods and kits for the rapid exponential amplification of nucleic acid molecules using a padlock probe. The present invention improves upon the existing methods for amplifying padlock probes by eliminating or delaying the appearance of artifact products that cause false positive results, and also increase the sensitivity and speed of the assay. Further provided are nucleic acid amplification primers containing non-informative base analogs.
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