Macromolecular protection assay
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United States of America Patent
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Issued Date -
May 6, 2004
app pub date -
Jul 25, 2003
filing date -
Jul 26, 2002
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a novel method for the detection and characterization of an unknown nucleic acid-binding protein from a biological sample. More particularly, the method of the instant invention includes the steps of forming nucleic acid-protein complexes, the subsequent selective degradation of of unbound nucleic acid molecules, the detection of nucleic acid-protein complexes, and finally, the characterization of the nucleic acid-binding protein. Additionally, compositions and methods are provided in the present invention for detecting and characterizing unknown nucleic acid-binding proteins from biological samples using immobilized nucleic acid molecules in reaction vessels having a multitude of wells.
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