Method for detecting and/or quantifying an analyte
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United States of America Patent
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Issued Date -
May 20, 2004
app pub date -
Dec 22, 2003
filing date -
Feb 12, 2001
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The invention relates to a method for detecting and/or quantifying a nucleic acid in a liquid. Said method comprises the following steps: a) either first microparticles and a probe having a specific affinity for the nucleic acid and for the first microparticles are prepared, or second microparticles are prepared having a probe which is bound to the surface thereof; b) a first solution containing the nucleic acid, the probe and the first microparticles is produced under conditions in which the probe binds to the nucleic acid and to the first microparticles, or a first solution containing the nucleic acid and the second microparticles is produced under conditions in which the nucleic acid binds to the probe; c) the first or second microparticles are separated from the first solution; and d) the nucleic acid is detected by means of an electrochemical method whereby the first or second microparticles are transferred into a second solution in order to detect the nucleic acid.
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