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Abstract

A microscope arrangement for fluorescence spectroscopy, especially fluorescence correlation spectroscopy, is proposed having at least two beam paths (38, 40, 50) that can be focused in each case onto a measuring volume, situated in a common measuring area of the microscope array, of a sample (12) to be investigated. At least one (50) of the beam paths (38, 40, 50) is an illuminating beam path that leads from a light source (44) to the measuring area. At least one further (38, 40) of the beam paths (38, 40, 50) is, moreover, an observing beam path that leads from the measuring area to a photodetector (26, 36) providing a fluorescence detection signal. The microscope arrangement has at least one optical element (18, 20, 22, 28, 30, 48) that is arranged in one of the beam paths (38, 40, 50) and can be adjusted for the purpose of setting the focus of this beam path (38, 40, 50). Provided according to the invention is an electronic actuating and control device (52) that responds to the fluorescence detection signal, is connected in an actuating fashion to the optical element (18, 20, 22, 28, 30, 48) and is designed to adjust the optical element (18, 20, 22, 28, 30, 48) as a function of the fluorescence detection signal in order to set the focus of the relevant beam path (38, 40, 50). A highly precise focal adjustment of the microscope arrangement is possible in this way.

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First Claim