Determination of analytes by means of fluorescence correlation spectroscopy
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United States of America Patent
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Issued Date -
Aug 5, 2004
app pub date -
Mar 29, 2004
filing date -
Mar 9, 2001
priority date (Note) -
Abandoned
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Abstract
The invention relates to a method for the determination of an analyte in a sample by fluorescence correlation spectroscopy, the distance between the measurement volume in the sample and the optical excitation/detection direction being .gtoreq.1 mm and the sample liquid being thermally insulated from the optical instrument. The method is especially suitable for the measurement of temperature-dependent processes, for example the determination of nucleic acid hybridization melting curves and/or for carrying out nucleic acid amplification reactions. A device suitable for carrying out the method according to the invention is furthermore disclosed.
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