Pre-incubation method to improve signal/noise ratio of nucleic acid assays
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United States of America Patent
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Issued Date -
Sep 16, 2004
app pub date -
Mar 14, 2003
filing date -
Mar 14, 2003
priority date (Note) -
Abandoned
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Abstract
A method for assaying a nucleobase-containing target with a nucleobase-containing probe, wherein: (a) the target is pre-incubated with at least one target incubation agent prior to being mixed with the probe; and/or (b) the probe is pre-incubated with at least one probe incubation agent prior to being mixed with the target to form the hybridization mixture. The pre-incubation enhances the signal to noise ratio of the assay. The pre-incubation medium and/or the hybridization medium can be pretreated with electric voltage. A kit for performing the method includes the probe, a label adapted to emit the signal, and at least one target incubation agent and/or probe incubation agent.
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