Methods and compositions for protein purification
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United States of America Patent
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Issued Date -
Sep 16, 2004
app pub date -
Jan 21, 2004
filing date -
May 15, 2001
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention provides methods of purifying proteins that include a metal ion affinity peptide. The methods generally involve contacting a fusion protein that includes a metal ion affinity peptide with at least two different metal ion chelating resins. In certain representative embodiments, the methods include contacting a fusion protein with a first metal ion chelate resin having a first immobilized metal ion; eluting any bound protein from the first metal ion chelate resin, to produce an eluate; contacting the eluate with a second metal ion chelate resin having a second immobilized metal ion; and eluting any bound protein from the second metal ion chelate resin. Also provided are kits for use in practicing the subject methods. The subject methods find use in a variety of protein purification applications.
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