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Abstract

A system and method of generating and acquiring phase contrast microscope images without interfering with the intensity and optical quality of other microscopy modalities employ wavelength-specific illumination and attenuation strategies for phase microscopy applications. A wavelength-specific objective phase ring that is opaque only at specific wavelengths may be used in conjunction with a phase microscopy apparatus. Attenuated wavelengths may be controlled such that opacity may be selectively provided only with respect to wavelengths that are outside of the desired range for the fluorescence signals being monitored. Illumination within the opaque wavelength range for the objective phase ring may be selected for phase microscopy applications. Accordingly, an objective phase ring effective for enabling wavelength-specific phase microscopy may not interfere with normal usage of the microscope for other applications such as, for example, fluorescence microscopy.

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First Claim