Methods of detecting differences in genomic sequence representation
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United States of America Patent
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Issued Date -
Sep 23, 2004
app pub date -
Jan 6, 2004
filing date -
Jan 10, 2003
priority date (Note) -
Abandoned
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Abstract
The invention relates to methods of genotyping the differential representation of one or more target genomic DNA sequences in a genomic DNA sample relative to a reference genomic DNA sample. The method uses tagged primer extension in which a set of tag sequences correspond to the identity of the elected target DNA sequence. Primer extension products are PCR amplified using a common set of tag-specific primers, the downstream primers bearing distinguishable labels. Following separation by size and/or charge, the detection of distinguishable label in a product of the anticipated size determines the identity and representation of the target DNA sequence in the sample. The method is well-suited for the genotyping of multiple target DNA sequence differences in one series of reactions.
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