Methods for using primers that encode one strand of a double-stranded promoter
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United States of America Patent
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Issued Date -
Oct 7, 2004
app pub date -
Nov 21, 2003
filing date -
Nov 21, 2002
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Abandoned
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Abstract
The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining circular single-stranded DNA transcription substrates using a promoter primer that encodes one strand of a double-stranded promoter. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to mRNA, making sense or anti-sense probes, detecting gene-or organism-specific sequences, or making RNAi.
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