Dual hybridization of complex nucleic acid samples for sequencing and single-nucleotide polymorphism identification
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United States of America Patent
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Issued Date -
Oct 14, 2004
app pub date -
Apr 14, 2003
filing date -
Apr 14, 2003
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Abstract
The present invention is drawn to a method of nucleic acid sequencing by hybridizing a selection probe with a conserved region of a target nucleic acid molecule that contains a sequence area of interest; hybridizing a sequencing probe that is different from the selection probe to a variable region of the target nucleic acid molecule; determining the nucleic acid sequence of the area of interest only. The present invention is for the identification and analysis of single nucleotide polymorphisms (SNP).
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