Method for selecting antibody expressing cells
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United States of America Patent
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Issued Date -
Nov 4, 2004
app pub date -
Jan 26, 2004
filing date -
Jul 27, 2001
priority date (Note) -
Abandoned
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Abstract
A method for selecting high level antibody producing cells, comprising the step of attaching protein A or protein G to a cell surface, thus capturing secreted, soluble antibody. Fluorescence staining of the captured antibody by conventional methods and subsequent FACS analysis allows to select low abundance high producer single cells.
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