Method for the production of chitin deacetylase
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United States of America Patent
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Issued Date -
Nov 18, 2004
app pub date -
Feb 12, 2004
filing date -
Feb 12, 2003
priority date (Note) -
Abandoned
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Abstract
In a first aspect, the present invention relates to a recombinant fungal strain, capable of expressing chitin deacetylase, which includes an expression vector that contains a nucleic acid molecule encoding chitin deacetylase, a suitable promoter and a transcription terminator. In particular, the present invention provides a recombinant Aspergillus oryzae strain capable of expressing chitin deacetylase obtained from Mucor rouxii. The present invention also relates to a method for producing chitin deacetylase by a recombinant fungal strain. In a second aspect, the present invention relates to a recombinant yeast strain, capable of expressing chitin deacetylase, which includes an expression vector that contains a nucleic acid molecule encoding chitin deacetylase, a suitable promoter and a transcription terminator. In particular, the present invention provides a recombinant Pichia pastoris strain capable of expressing chitin deacetylase obtained from Mucor rouxii. The present invention also relates to a method for producing chitin deacetylase by a recombinant yeast strain. The present invention further relates to purified recombinant chitin deacetylase enzyme.
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