Development and stratification of pine somatic embryos using a liquid system

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United States of America Patent

PATENT NO 7732205
SERIAL NO

10875656

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Abstract

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The invention provides methods for producing pine somatic embryos using a liquid development medium and/or a liquid stratification medium. In a first aspect, the methods comprise the step of culturing embryogenic cells in, or on, a liquid development medium to produce cotyledonary pine somatic embryos. In another aspect, the methods comprise the step of culturing pine cotyledonary somatic embryos in, or on, a liquid stratification medium to produce stratified cotyledonary somatic embryos. The invention also provides methods for producing pine somatic embryos in bioreactors.

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Patent Owner(s)

  • WEYERHAEUSER NR COMPANY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gupta, Pramod K Federal Way, US 37 591
Holmstrom, Diane G Sumner, US 8 17
Larson, Bonnie Granite Falls, US 10 24
Zucati, Judith Maple Valley, US 1 3

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