Characterising polypeptides
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United States of America Patent
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Issued Date -
Feb 24, 2005
app pub date -
Oct 18, 2004
filing date -
Jun 7, 2001
priority date (Note) -
Abandoned
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Abstract
Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap .epsilon.-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block .alpha.-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.
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