Expression of membrane proteins using an adenylyl cyclase of mycobacterium tube rculosis
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United States of America Patent
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Issued Date -
Mar 3, 2005
app pub date -
Apr 29, 2004
filing date -
May 14, 2001
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Abandoned
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Abstract
A method for expression of membrane proteins is provided, wherein at least a portion of a nucleotide sequence coding to the membrane protein is fused to at least a portion of a nucleotide sequence coding for an adenylyl cyclase of Mycobacterium tuberculosis or a sequence at least about 70% identical to said sequence of Mycobacterium tuberculosis. In a second step the fused nucleotide sequence is introduced into an organism and/or a living cell. Another aspect of the invention is a method for searching active agents, which interact directly with adenylyl cyclases.
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