Methods and kit for hybridization anaylsis using peptide nucleic acid probes
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United States of America Patent
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Issued Date -
Mar 10, 2005
app pub date -
Aug 7, 2003
filing date -
Oct 6, 1995
priority date (Note) -
Abandoned
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Abstract
A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.
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