siRNA encoding constructs and methods for using the same
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United States of America Patent
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Issued Date -
Mar 17, 2005
app pub date -
Aug 27, 2004
filing date -
Sep 11, 2003
priority date (Note) -
Abandoned
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Abstract
siRNA encoding constructs and methods for using the same are provided. The subject constructs are characterized by including a siRNA coding domain flanked by opposing promoters. In using the subject constructs, sense and antisense strands of the desired siRNA product encoded by the coding domain are transcribed under the direction of the two opposing promoters flanking the coding domain. The transcribed sense and antisense strands are then annealed to each other to produce the desired siRNA double-stranded molecule. The subject constructs and methods find use in a variety of applications, including applications where the specific reduction or silencing of a gene is desired. Also provided are systems and kits for use in practicing the subject invention.
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