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Abstract

The present invention relates to a method for the quantitative determination of the amount of one or more biomolecules, such as proteins or polypeptides, in one or more samples by utilising sample unique tagging reagents. More specifically, the method comprises steps of providing at least two samples; reacting biomolecules present in each sample with a sample unique mass tagging reagent to provide sample unique mass tagged forms thereof; combining tagged forms present in each sample to provide a single sample; coseparating, from the resulting sample, a mix of mass tagged forms of each of said biomolecules into different fractions; subjecting, for each fraction, the mix to mass spectrometry to obtain a mass spectrum; and determining from signals in each mass spectrum, the amount of the biomolecule corresponding to the spectrum in at least one of said samples relative to the amount of the same biomolecule in at least one of the remaining samples. In an advantageous embodiment, the separation step is a gel electrophoresis step. In some cases, it may be advantageous to also include a step of digesting biomolecules, such as protein(s).

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First Claim