3D format biochips and method of use
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United States of America Patent
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Issued Date -
May 12, 2005
app pub date -
Dec 16, 2004
filing date -
Oct 26, 2000
priority date (Note) -
Abandoned
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Abstract
A biochip is formed with a plurality of optically clear hydrogel microspots attached to the top surface of a solid substrate in the form of an array. Each of the microspots is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities or probes are immobilized in these microspots, which entities are effective to selectively hybridize to or sequester target biomolecules, such as target cells. Different binding entities are immobilized in microspots in different regions of an array to create a biochip that can be used to assay for or separate a number of target biomolecules, such as cells from maternal blood.
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