Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication
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United States of America Patent
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Issued Date -
Jul 7, 2005
app pub date -
Dec 30, 2003
filing date -
Dec 30, 2003
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Abandoned
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Abstract
The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.
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Patent Owner(s)
Patent Owner | Address | |
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INTEL CORPORATION | 2200 MISSION COLLEGE BOULEVARD SANTA CLARA CA 95054 |
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Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Berlin, Andrew | San Jose, CA | 38 | 1623 |
Chan, Selena | San Jose, CA | 60 | 1846 |
Koo, Tae-Woong | South San Francisco, CA | 71 | 1790 |
Su, Xing | Cupertino, CA | 187 | 4616 |
Sun, Lei | Santa Clara, CA | 325 | 2326 |
Sundararajan, Narayanan | San Francisco, CA | 32 | 894 |
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Fee | Large entity fee | small entity fee | micro entity fee |
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Surcharge after expiration - Late payment is unavoidable | $700.00 | $350.00 | $175.00 |
Surcharge after expiration - Late payment is unintentional | $1,640.00 | $820.00 | $410.00 |
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