Importance

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Abstract

A biosensor (20) for indicating electrochemically the catalytic activity of an enzyme in the presence of a biological fluid containing an analyte acted upon by said enzyme comprises: (a) a first substrate (2); (b) a second substrate (18) overlying at least a part of the first substrate (2); (c) a working electrode (24) on one of the substrates, the working electrode (24) including a catalytically-active quantity of said enzyme; (d) a counter electrode (22) on one of the substrates; (e) conductive tracks (4, 6) connected to said working (24) and counter (22) electrodes for making electrical connections with a test meter apparatus; (f) a spacer layer (14) having a channel (16) therein and disposed between the first substrate (2) and the second substrate (18), the spacer layer channel (16) co-operating with adjacent surfaces to define a capillary flow path which extends from an edge of at least one of said substrates (2, 18) to said electrodes (22, 24); wherein the electrodes (22, 24) are arranged such that a fluid sample which flows along the capillary flow path from said edge will substantially completely cover the working electrode (24) before the fluid sample makes contact with any part of the counter electrode (22).

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First Claim