Importance

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Abstract

A non-mediated biosensor for indicating amperometrically the catalytic activity of an oxidoreductase enzyme in the presence of a fluid containing a substance acted upon by said enzyme, the biosensor comprising: (a) a first substrate; (b) a working electrode and a counter electrode on the first substrate; (c) conductive tracks connected to said electrodes for making electrical connections with a test meter apparatus; (d) a second substrate overlying at least a part of the first substrate; and (e) a spacer layer having a channel therein and disposed between the first substrate and the second substrate, the spacer layer channel co-operating with adjacent surfaces to define a capillary flow path which does not contain a mesh and which extends from an edge of at least one of said substrates to said electrodes; wherein the working electrode includes: (f) an electrically-conductive base layer comprising particles of finely divided platinum-group metal or platinum-group metal oxide bonded together by a resin; (g) a top layer on the base layer, said top layer comprising a buffer; and (h) a catalytically-active quantity of said oxidoreductase enzyme in at least one of said base layer and said top layer.

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First Claim