Method for determining oligonucleotide concentration
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United States of America Patent
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Issued Date -
Jul 14, 2005
app pub date -
Feb 3, 2005
filing date -
May 29, 2002
priority date (Note) -
Abandoned
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Abstract
The invention provides a simple, sensitive method to determine the concentration of oligonucleotides in biological matrices, such as plasma. The method involves obtaining a biological specimen containing a concentration of an oligonucleotide, removing an amount of protein from the sample, subjecting the sample to a chromatographic separation, and analyzing the eluant for the amount of oligonucleotide by mass spectrometry. The assay provides a reliable measure of the concentration of oligonucleotide in the concentration range of about 5 to about 10,000 ng/mL of sample.
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