Synthetic and recombinant substrates for the detecion of the von willebrand factor-cleaving protease
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United States of America Patent
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Issued Date -
Jul 14, 2005
app pub date -
Sep 18, 2003
filing date -
Jul 28, 2000
priority date (Note) -
Abandoned
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Abstract
A method of detecting von Willebrand Factor-cleaving protease in a test sample is disclosed. In one embodiment, the method comprises the steps of (a) obtaining a test bodily fluid sample; (b) exposing the test sample to a monomeric von Willebrand Factor fragment, wherein the fragment comprises amino acids 842 and 843, wherein cleavage of the fragment will occur proportional to the amount of protease in the sample; and (c) comparing the cleavage products to a standard curve and determining the amount of von Willebrand Factor-cleaving protease in the test sample.
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