US Patent Application No: 2005/0196,755

Number of patents in Portfolio can not be more than 2000

In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

The present invention relates to a high efficiency method of expressing immunoglobulin molecules in eukaryotic cells. The invention is further drawn to a method of producing immunoglobulin heavy and light chain libraries, particularly using the trimolecular recombination method, for expression in eukaryotic cells. The invention further provides methods of selecting and screening for antigen-specific immunoglobulin molecules, and antigen-specific fragments thereof. The invention also provides kits for producing, screening and selecting antigen-specific immunoglobulin molecules. Finally, the invention provides immunoglobulin molecules, and antigen-specific fragments thereof, produced by the methods provided herein.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddressTotal Patents
UNIVERSITY OF ROCHESTERROCHESTER, NY391

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Smith, Ernest S Ontario, NY 30 107
Zauderer, Maurice Pittsford, NY 40 202

Cited Art Landscape

Patent Info (Count) # Cites Year
 
Other [Check patent profile for assignment information] (1)
* 2004/0265,900 T cells specific for target antigens and methods and vaccines based thereon 2 2004
 
DGI BIOTECHNOLOGIES, INC. (1)
* 6,010,861 Target specific screens and their use for discovering small organic molecular pharmacophores 8 1995
 
XOMA TECHNOLOGY LTD. (3)
* 5,618,920 Modular assembly of antibody genes, antibodies prepared thereby and use 154 1994
* 5,576,195 Vectors with pectate lyase signal sequence 37 1994
* 5,693,493 Modular assembly of antibody genes, antibodies prepared thereby and use 54 1995
 
SCHERING CORPORATION (1)
* 4,642,334 Hybrid DNA prepared binding composition 96 1983
 
BIOFOCUS DISCOVERY LIMITED (1)
* 6,297,004 Recombinant viruses displaying a nonviral polypeptide on their external surface 23 1998
 
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM (1)
* 2004/0072,740 Directed evolution of enzymes and antibodies 5 2001
 
Dyax Corp. (4)
* 5,223,409 Directed evolution of novel binding proteins 1193 1991
* 5,571,698 Directed evolution of novel binding proteins 437 1993
* 5,837,500 Directed evolution of novel binding proteins 164 1995
* 2003/0186,374 Multi-chain eukaryotic display vectors and uses thereof 56 2002
 
DOMANTIS LIMITED (1)
* 6,696,245 Methods for selecting functional polypeptides 32 1998
 
NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT (2)
* 7,015,024 Compositions containing recombinant poxviruses having foreign DNA expressed under the control of poxvirus regulatory sequences 2 1995
* 6,998,252 Recombinant poxviruses having foreign DNA expressed under the control of poxvirus regulatory sequences 4 1995
 
The Scripps Research Institute (2)
* 6,291,160 Method for producing polymers having a preselected activity 42 1992
* 5,959,177 Transgenic plants expressing assembled secretory antibodies 64 1996
 
Exxon Chemical Patents Inc. (1)
* 5,565,332 Production of chimeric antibodies - a combinatorial approach 432 1994
 
UNIVERSITY OF ROCHESTER (11)
* 6,872,518 Methods for selecting polynucleotides encoding T cell epitopes 7 1997
* 2003/0133,917 T CELLS SPECIFIC FOR TARGET ANTIGENS AND METHODS AND VACCINES BASED THEREON 0 1997
* 7,067,251 Methods of directly selecting cells expressing inserts of interest 4 2001
* 2002/0018,785 Methods for producing polynucleotide libraries in vaccinia virus 0 2001
* 6,706,477 Methods for producing polynucleotide libraries in vaccinia virus 6 2001
* 2002/0123,057 In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells 33 2001
* 6,800,442 Methods of selecting polynucleotides encoding antigens 6 2002
* 2003/0104,402 Methods of producing or identifying intrabodies in eukaryotic cells 3 2002
* 2002/0192,675 Methods of identifying regulator molecules 5 2002
* 2003/0194,696 Methods of producing a library and methods of selecting polynucleotides of interest 8 2002
* 2006/0160,129 Methods of producing a library and methods of selecting polynucleotides of interest 2 2006
 
MEDICAL RESEARCH COUNCIL, OF (1)
* 5,830,663 In situ recombinant PCR within single cells 47 1994
 
Health Research, Inc. (4)
* 4,769,330 Modified vaccinia virus and methods for making and using the same 148 1981
* 4,603,112 Modified vaccinia virus 178 1982
* 5,110,587 Immunogenic composition comprising synthetically modified vaccinia virus 82 1990
* 5,972,597 Methods using modified vaccinia virus 6 1995
 
BIOSITE INCORPORATED (2)
* 6,057,098 Polyvalent display libraries 114 1997
* 6,420,113 Chimeric polyclonal antibodies 18 1999
 
GENVEC, INC. (1)
* 6,861,229 Method of identifying a gene product 3 2003
 
Genetastix Corporation (1)
* 6,410,246 Highly diverse library of yeast expression vectors 33 2000
 
ROCHE MOLECULAR SYSTEMS, INC. (3)
* 4,800,159 Process for amplifying, detecting, and/or cloning nucleic acid sequences 894 1986
* 4,965,188 Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme 1186 1987
* 4,889,818 Purified thermostable enzyme 370 1987
 
ATLANTIC PRECAST CONCRETE INC. (1)
* 5,932,255 Apparatus for casting wire reinforced members utilizing members for positioning and supporting the wire reinforcements 2 1997
 
MEDICAL RESEARCH COUNCIL (4)
* 5,225,539 Recombinant altered antibodies and methods of making altered antibodies 673 1991
* 5,871,907 Methods for producing members of specific binding pairs 134 1994
* 5,885,793 Production of anti-self antibodies from antibody segment repertoires and displayed on phage 163 1994
* 5,733,743 Methods for producing members of specific binding pairs 320 1994
 
JAPAN TOBACCO INC. (2)
* 6,130,364 Production of antibodies using Cre-mediated site-specific recombination 93 1995
* 5,916,771 Production of a multimeric protein by cell fusion method 150 1996
 
MEDICAL RESEARCH COUNCIL OF UNITED KINGDOM (1)
* 6,248,516 Single domain ligands, receptors comprising said ligands methods for their production, and use of said ligands and receptors 62 1995
 
STRATAGENE CALIFORNIA (4)
* 6,291,158 Method for tapping the immunological repertoire 77 1992
* 6,291,159 Method for producing polymers having a preselected activity 30 1992
* 6,291,161 Method for tapping the immunological repertiore 46 1992
* 6,635,424 Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules 5 2001
 
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY (2)
* 4,656,134 Gene amplification in eukaryotic cells 94 1985
* 5,807,715 Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin 220 1994
 
MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH (1)
* 5,023,171 Method for gene splicing by overlap extension using the polymerase chain reaction 128 1989
 
CATALYST ASSETS LLC (2)
* 5,780,225 Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules 164 1994
* 6,479,243 Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules 8 2001
 
CELLTECH LIMITED (2)
* 4,816,397 Multichain polypeptides or proteins and processes for their production 570 1984
* 5,965,405 Method for producing Fv fragments in eukaryotic cells 13 1993
 
VRIJE UNIVERSITEIT BRUSSEL (2)
* 5,759,808 Immunoglobulins devoid of light chains 46 1995
* 5,800,988 Immunoglobulins devoid of light chains 38 1995
 
Pasteur Mérieux Sérums et Vaccins S.A. (1)
* 6,599,697 Process for preparing a multicombinatorial library of vectors for expressing antibody genes 9 1996
 
VACCINEX, INC. (1)
* 2005/0266,425 Methods for producing and identifying multispecific antibodies 4 2004
 
IMMUNO AKTIENGESELLSCHAFT (2)
* 5,445,953 Direct molecular cloning of a modified poxvirus genome 10 1991
* 5,770,212 Recombinant poxviruses with foreign DNA in essential regions 30 1997
 
TRUSTEES OF BOSTON UNIVERSITY (2)
* 5,789,208 Polyclonal antibody libraries 71 1997
* 6,335,163 Polyclonal antibody libraries 31 1998
 
Genentech, Inc. (1)
* 6,331,415 Methods of producing immunoglobulins, vectors and transformed host cells for use therein 245 1988
 
LAWRENCE LIVERMORE NATIONAL SECURITY, LLC (1)
* 6,055,079 Optical key system 18 1997
 
ABBVIE BIOTECHNOLOGY LTD. (1)
* 7,223,394 Human antibodies that bind human TNF.alpha. 81 2001
 
GILLIES, STEPHEN D. (1)
* 5,650,150 Recombinant antibody cytokine fusion proteins 84 1994
 
PRESIDENT AND FELLOWS OF HARVARD COLLEGE (1)
* 4,946,786 T7 DNA polymerase 29 1990
* Cited By Examiner

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
VACCINEX, INC. (2)
7,919,095 Anti-IL-6 monoclonal antibodies 0 2007
8,637,026 Anti-C35 antibody combination therapies and methods 0 2008
 
ABBVIE BIOTHERAPEUTICS INC. (3)
7,947,495 Immunoglobulin display vectors 1 2008
* 8,633,139 Methods of screening complex protein libraries to identify altered properties 0 2008
8,283,170 Immunoglobulin display vectors 0 2011
 
UNIVERSITY OF ROCHESTER (8)
* 7,858,559 In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells 1 2001
7,563,882 Polynucleotides encoding antibodies that bind to the C35 polypeptide 20 2003
* 2004/0063,907 Gene differentially expressed in breast and bladder cancer and encoded polypeptides 4 2003
8,741,810 In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells 0 2007
7,750,125 Antibodies that bind to the C35 polypeptide 15 2007
7,968,688 Antibodies that bind to the C35 polypeptide 0 2009
7,879,990 Polynucleotides encoding antibodies that bind to the C35 polypeptide 0 2009
7,910,551 Gene differentially expressed in breast and bladder cancer and encoded polypeptides 0 2009
 
The Board of Trustees of the University of Illinois (3)
7,465,787 Yeast cell surface display of proteins and uses thereof 11 2003
7,569,357 High affinity TCR proteins and methods 3 2004
8,372,636 Yeast cell surface display of proteins and uses thereof 0 2008
 
CRUCELL HOLLAND B.V. (3)
* 8,642,513 Method for preparing immunoglobulin libraries 1 2006
8,211,431 Human binding molecules having killing activity against staphylococci and uses thereof 2 2007
8,460,666 Human binding molecules having killing activity against staphylococci and uses thereof 1 2012
 
AMGEN INC. (2)
7,884,054 Methods for identifying functional antibodies 1 2004
* 2005/0282,181 Methods for identifying functional antibodies 13 2004
 
MUSC FOUNDATION FOR RESEARCH DEVELOPMENT (1)
8,715,743 Human monoclonal antibodies and methods for producing the same 0 2008
 
JANSSEN BIOTECH, INC. (3)
* 8,663,980 Vectors, host cells, and methods of production and uses 1 2008
* 2011/0008,321 Vectors, Host Cells, and Methods of Production and Uses 0 2008
8,980,626 Vectors, host cells, and methods of production and uses 0 2014
 
4-ANTIBODY AG (3)
7,741,077 Method for the generation of genetically modified vertebrate precursor lymphocytes and use thereof for the production of heterologous binding proteins 3 2001
8,748,353 Identification of antigen or ligand-specific binding proteins 0 2009
8,716,194 Identification of antigen or ligand-specific binding proteins 0 2011
* Cited By Examiner