Recombinant icosahedral virus like particle production in pseudomonads
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United States of America Patent
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Issued Date -
Sep 29, 2005
app pub date -
Dec 1, 2004
filing date -
Dec 1, 2003
priority date (Note) -
Abandoned
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Abstract
The present invention provides an improved process for the production of recombinant peptides by fusion of recombinant peptides with icosahedral viral capsids and expression of the fusion in bacterial cells of Pseudomonad origin. The Pseudomonad cells support formation of virus like particles from icosahedral viral capsids in vivo, and allow the inclusion of larger recombinant peptides as monomers or concatamers in the virus like particle. The invention specifically provides cells expressing viral capsid fusions, nucleic acids encoding fusions of toxic proteins with icosahedral viral capsids and processes for manufacture of recombinant proteins.
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