Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
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United States of America Patent
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Apr 13, 2010
Grant Date -
Sep 29, 2005
app pub date -
Mar 3, 2005
filing date -
Sep 5, 2002
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Abstract
The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.
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