Multidimensional separations employing an array of electrophoresis channels
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United States of America Patent
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Issued Date -
Oct 6, 2005
app pub date -
Apr 12, 2005
filing date -
Jun 6, 2000
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a method for separating components of a sample. The method includes obtaining a first separation of the sample components along a first dimension wherein the sample components are at least partially resolved, wherein the first separation can be performed in the absence of an electric field applied to the first dimension. An electric field is used to obtain a second separation of the sample components along a second dimension comprising a plurality of substantially isolated volumes. An intensity-time data record is obtained from each of the isolated volumes, the intensity-time data records containing peaks, each peak being indicative of a migration time. The migration time of a first peak is normalized with respect to a migration time of at least a second peak to correct for migration time differences between the isolated volumes.
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