Optically-detectable enzyme substrates and their method of use
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United States of America Patent
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Issued Date -
Oct 13, 2005
app pub date -
Jan 21, 2005
filing date -
Jan 21, 2004
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a .beta.-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a .beta.-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a .beta.-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a .beta.-lactmase or instructions for using the components in an assay.
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