PCR amplification reaction apparatus and method for PCR amplification reaction using apparatus
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United States of America Patent
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Aug 4, 2009
Grant Date -
Oct 27, 2005
app pub date -
Apr 22, 2005
filing date -
Apr 26, 2004
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Abstract
The present invention provides a method for PCR amplification reaction that can reduce the time required for amplification reaction of nucleic acid by PCR using the target nucleotide molecule as a template, and an apparatus used for the method. The method comprises using a PCR reaction vessel in which a heater for local heating, which can thermally contact with a solution storing section with an extremely small capacity for storing a PCR reaction solution and a reaction solution stored in the solution storing section directly, is placed; feeding a pulsed current to the placed heater; and carrying out a thermal cycle of PCR reaction, so that high-speed PCR amplification reaction can be carried out.
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