Quantitative multiplex amplification on a genomic scale, and applications for detecting genomic rearrangements
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United States of America Patent
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Issued Date -
Nov 3, 2005
app pub date -
Apr 18, 2005
filing date -
Jul 19, 2002
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Abstract
The present application relates to novel composite primers which make it possible to amplify in multiplex at a quantitative level of precision, and to the application of these composite primers for detecting gnomic rearrangements in general and cryptic chromosomal rearrangements in particular. These composite primers contain a tag the sequence of which is absent from or poorly represented in the genome analyzed, and which exhibits a very low propensity to form stable pairings. The composite primers, which contain them, make it possible to carry out multiplex amplifications with quantitative precision on the scale of a genome such as the human genome.
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