Methods and compositions for increasing viral vector production in packaging cell lines
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United States of America Patent
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app pub date -
Jan 18, 2005
filing date -
Apr 30, 1999
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Abandoned
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Abstract
Methods and compositions are disclosed to increase viral titer of vector producing cell lines and to reduce potential for re-infection by inhibiting deactivation of helper virus. According to the invention, methylation of helper virus and concomitant helper virus inactivation is directly correlated with increased super-infection and decreased vector production. Novel helper virus, packaging and producing cells and viral vectors are disclosed with improved safety and stability by decreasing helper virus inactivation.
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