Gel for electrophoresis and method for preparing the same

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United States of America Patent

SERIAL NO

11210767

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Abstract

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The present invention herein provides a gel for electrophoresis which comprises a gel prepared from single-stranded glucan which has .beta.-1,3-glucoside bonds as a main chain and .beta.-1,6-glucoside bonds on side chains, or a mixture of the glucan with a second polysaccharide, or acrylamide and/or derivative thereof; and a method for the preparation of a gel for electrophoresis comprising the steps of dissolving a glucan, which has .beta.-1,3-glucoside bonds as a main chain and .beta.-1,6-glucoside bonds on side chains, in a first solvent which can dissociate the helical structure of the glucan to thus form single-stranded glucan; removing the first solvent; dissolving the single-stranded glucan in a second solvent capable of dissolving the same; and then heat-treating the resulting solution. The pore size of the gel according to the present invention can easily be controlled, the gel permits the isolation and/or analysis of a nucleic acid or a protein whose molecular weight falls within a wider range and the gel never interferes with the detection, through the staining technique, of such a nucleic acid or a protein separated and developed on or within the gel.

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Patent Owner(s)

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MITSUI SUGAR CO LTDTOKYO 13-8423

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kawaguchi, Makiko Kobe-shi, JP 1 0
Sasaki, Yohei Kobe-shi, JP 4 7
Yoneta, Masahiko Osaka-shi, JP 5 166

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