Viral libraries from uncultivated viruses and polypeptides produced therefrom
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United States of America Patent
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Issued Date -
Dec 22, 2005
app pub date -
Jun 15, 2005
filing date -
Jun 17, 2004
priority date (Note) -
Abandoned
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Abstract
Provided is a method for producing viral genomic and complementary DNA expression libraries and novel viral polypeptides without requiring virus cultivation prior to library construction. The method includes direct isolation of viral particles from the environment by differential filtration and centrifugation. The viral nucleic acids are extracted and used to construct libraries that may be screened by one of several methods to identify useful coding sequences or may be used to produce novel polypeptides.
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